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目的:研究睾丸去神经支配对性激素水平的影响,探讨精索神经对精子发生的调控机制。方法:健康成年雄性SD大鼠48只,随机分为假手术组(SO)、精索上神经切除组(SSN)、精索下神经切除组(ISN)和精索上神经+精索下神经切除组(SSN+ISN),每组12只大鼠。解剖显微镜下建立睾丸去神经支配大鼠模型,分别于术前7天、术后1、7、14、21、28天于大鼠眼眶后静脉丛处取血,放射性免疫的方法检测血清中睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)、泌乳素(PRL)和雌二醇(E2)水平。结果:术后1天各组血清T水平均显著下降(P<0.05),术后7天回升,此后去神经组T水平持续下降(P<0.05),SSN+ISN组较SSN组和ISN组下降更为显著(P<0.05),SO组无明显变化(P>0.05);术后1天各组血清LH水平均显著升高(P<0.05),术后7天稍有回落,此后去神经组LH水平持续上升,SSN+ISN组较SSNG和ISN组上升更为显著,SO组无明显变化(P>0.05);各组血清中FSH水平均于术后1天显著升高(P<0.05),后逐渐恢复至术前水平;去神经组血清中PRL水平于手术后显著升高(P<0.05),SO组无明显改变(P>0.05);术后去神经组血清E2水平均显著下降(P<0.05),SO组仅术后1天显著下降(P<0.05),后恢复到术前水平(P>0.05)。结论:精索神经对多种性激素水平具有调节作用,这可能是其实现生精调控作用的重要途径之一。
Objective: To study the effect of testicular denervation on sex hormone levels and to explore the regulation mechanism of spermatogenesis on spermatic nerve. Methods: Forty-eight healthy adult male Sprague-Dawley rats were randomly divided into four groups: sham operation group (SO), SSN group, ISN group, and superior spermatic cord The resection group (SSN + ISN), 12 rats per group. The model of testicular denervation in rats was established by dissecting microscope. The blood was collected at 7, 7, 14, 21 and 28 days after operation in rats, and the levels of testosterone in serum were measured by radioimmunoassay (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and estradiol (E2). Results: At 1 day after operation, serum T level decreased significantly (P <0.05) and recovered at 7 days postoperatively. T level in denervating group continued to decrease (P <0.05). Compared with SSN group and ISN group (P <0.05). The level of serum LH in each group was significantly increased (P <0.05) at 1 day after operation and slightly decreased at 7 days after operation The level of LH in the neurological group continued to rise, the level of SSH + ISN was more significant than that in SSNG and ISN group, but there was no significant change in SO group (P> 0.05). The levels of serum FSH in each group were significantly increased at 1 day after operation (P < 0.05), and then gradually returned to the preoperative level. The level of PRL in the serum of denervating group was significantly increased after operation (P <0.05), but not in SO group (P> 0.05) (P <0.05). However, SO group only decreased significantly (P <0.05) one day after surgery and returned to preoperative level (P> 0.05). Conclusion: The spermatic nerve plays a regulatory role in the regulation of various sex hormones, which may be one of the important ways to achieve the regulation of spermatogenesis.