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目的:研究细胞周期蛋白E2(Cyclin E2)在人鼻咽癌(NPC)组织中的表达及临床意义。方法:应用免疫组织化学法和RT-PCR法检测NPC组织及鼻咽非肿瘤组织Cyclin E2蛋白和mRNA的表达。结果:Cyclin E2蛋白在NPC和鼻咽非肿瘤组织中的表达率分别为75%(45/60)和9.5%(2/21),两者相比有统计学意义(P<0.01);鼻咽癌组织中Cyclin E2蛋白的过表达对同年龄、性别的患者无统计学差异(P>0.05),但与淋巴结转移、分期和5年生存率有相关性(P<0.01)。鼻咽癌组与对照组相比,Cyclin E2 mRNA表达亦有统计学差异(P<0.01)。结论:Cyclin E2表达对判断鼻咽癌病变进展、生存率及转移有重要参考价值。
Objective: To study the expression and clinical significance of cyclin E2 in human nasopharyngeal carcinoma (NPC). Methods: The expressions of Cyclin E2 protein and mRNA in NPC tissues and nasopharyngeal non-tumor tissues were detected by immunohistochemistry and RT-PCR. Results: The expression rates of Cyclin E2 protein in NPC and nasopharyngeal non-tumor tissues were 75% (45/60) and 9.5% (2/21), respectively, with statistical significance (P <0.01) The overexpression of Cyclin E2 protein in pharyngeal carcinoma showed no significant difference between the same age and gender (P> 0.05), but correlated with lymph node metastasis, staging and 5-year survival rate (P <0.01). Compared with the control group, the expression of Cyclin E2 mRNA in nasopharyngeal carcinoma group was also significantly different (P <0.01). Conclusion: The expression of Cyclin E2 has an important reference value for judging the pathological changes, survival rate and metastasis of nasopharyngeal carcinoma.