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正常的成纤维或上皮细胞,在体外培养过程中可能发生转化(transformation)。转化可因致癌化合物或致瘤病毒处理培养物而产生,也可能自发产生。两种类型的转化特点在许多方面是类似的,主要有细胞接种于敏感动物有致瘤性,细胞具有无限生长期(永生性)丧失停泊依赖性,对血清的依赖性降低,被外源性凝集因子的凝集能力增加,对化学致癌的毒性作用的抗性增加,对培养液内钙离子含量增加而增殖的依赖性降低,纤粘素合成下降等。
Normal fibroblasts or epithelial cells may undergo transformation during in vitro culture. Transformation can be caused by oncogenic compounds or oncolytic virus-treated cultures and may also occur spontaneously. The two types of transformational features are similar in many respects, principally with tumorigenicity of cells inoculated in sensitive animals, loss of anchorage dependence of immortalized cells (immortalized), reduced dependence on serum, exogenous agglutination The agglutination ability of the factor is increased, the resistance to the toxic effect of the chemical carcinogen is increased, the dependence on the proliferation of the calcium ion content in the culture solution is reduced, and the synthesis of the fibronectin is decreased.