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研究了固相萃取-高效液相色谱法测定人血清中左甲状腺素钠的方法。血清样品经MCX固相萃取小柱提取后进样测定;色谱条件为:色谱柱ODS柱(150 mm×4.6 mm i.d.,4.6μm),流动相为甲醇:H3PO4溶液(60:40,V:V,pH 3.00);流速为1.0 mL/min,检测波长225 nm,柱温30℃,进样量20μL。在0.005~10.0μg/mL浓度范围内,关系线性关系良好;检出限为0.002μg/mL;RSD<6.4%,回收率在96.6%~104.4%之间。方法适用于左甲状腺素钠的人体药代动力学及生物利用度研究。
A method for the determination of levothyroxine in human serum by solid-phase extraction and high performance liquid chromatography was studied. Serum samples were extracted by MCX solid phase extraction cartridges. The chromatographic conditions were as follows: column ODS column (150 mm × 4.6 mm id, 4.6 μm) with methanol: H3PO4 (60:40, V: V , pH 3.00). The flow rate was 1.0 mL / min, the detection wavelength was 225 nm, the column temperature was 30 ℃ and the injection volume was 20 μL. The linear range was 0.005 ~ 10.0μg / mL. The detection limit was 0.002μg / mL. The RSD was less than 6.4% and the recovery was between 96.6% and 104.4%. The method is suitable for the study of human pharmacokinetics and bioavailability of levothyroxine sodium.