肝癌TIL细胞转导IL-2基因的生物学变化研究

• 作 者: 李东复 王树和 太京华 张国荣
• 机 构: 白求恩医科大学第二临床学院消化内科,白求恩医科大学基础医学院免疫教研室
• 出 处: 中国免疫学杂志
• 发表日期: 1999年07期
• 关键词: 细胞转导 TIL细胞 基因转移 逆转录病毒载体 人肝 基因图 病毒载体介导 生物学变化 细胞生长 癌浸润

目的：为探讨ＩＬ２基因导入肝癌浸润淋巴细胞及对其生物学活性的影响。方法：应用逆转录病毒载体介导ＩＬ２基因导入人肝癌浸润淋巴细胞（ＴＩＬ），同时对经转导ＩＬ２基因的肝癌浸润淋巴细胞的杀伤活性，增殖能力及表型等进行了观察。结果：转导ＩＬ２基因后的肝癌浸润淋巴细胞能表达一定量的ＩＬ２，明显高于未转导细胞，但其杀伤肿瘤细胞活性，增殖能力及表型等没有显著变化。结论：应用逆转录病毒载体介导ＩＬ２基因导入人肝癌浸润淋巴细胞获得成功，亦有ＩＬ２基因产物表达。

Objective: To investigate the effect of IL2 gene infiltrating hepatoma infiltrating lymphocytes and their biological activities. METHODS: The retroviral vector-mediated IL2 gene was introduced into human hepatoma infiltrating lymphocytes (TIL). At the same time, the killing activity, proliferative capacity and phenotype of hepatoma infiltrating lymphocytes transduced with IL2 gene were observed. . Results: The infiltrating lymphocytes of hepatoma after transduction of IL-2 gene could express a certain amount of IL-2, which was significantly higher than that of non-transduced cells, but its activity, proliferation ability and phenotype of tumor cells were not significantly changed. Conclusion: The retroviral vector-mediated IL2 gene was successfully introduced into human hepatoma infiltrating lymphocytes, and IL2 gene products were also expressed.