Conformational changes of active site of copper zinc superoxide dismutase can be detected sensitivel

• 作 者: 舒占永
• 机 构: StateKeyLaboratoryofBiomacromolecules,InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101,C
• 出 处: 中国科学：生命科学英文版
• 发表日期: 1996年6期
• 关键词: CUZN-SOD DENATURATION conformational changes of active SITE ELECTRON-TRANSFER (E
• 基金项目: Project supported by China Postdoctoral Science Foundation and the National Natural Science Foundation of China.Abbteviation: CuZn-SOD,copper zinc superoxide dismutase, ET, electron-transfer,GuHCl,gua

The electron-transfer (ET) reaction between Fe(CN)64- and copper zinc superoxide dismutase (CuZn-SOD) occurs at the active site of the enzyme. The ET parameters which are sensitive to the denaturation have been used to determine the conformational changes