Fusion expression of mutated cecropin CMIV in E. coli

• 作 者: 谢维 邱奇峰 董雪吟 华子春 徐贤秀
• 机 构: StateKeyLaboratoryofPharmaceuticalBiotechnology,DepartmentofBiochemistry,NanjingUniversity,Nanjing21
• 出 处: 中国科学：生命科学英文版
• 发表日期: 1997年3期
• 关键词: CECROPIN FUSION EXPRESSION E. coli. cecropin fusion expression E. coli.

A cDNA coding mutated cecropin CMIV from Bombyx mori was synthesized according to its amino acid sequense using E .coli biased codons .The gene was cloned into the fusion expression vector pEZZ318 and was expressed in E .coli HB101.The fusion protein prod