论文部分内容阅读
自1906年Harrison创建细胞培养技术以来,在各学科领域内得到了广泛应用,尤其近年来在毒理学中的应用更引人注目,已逐渐形成了一门新的学科-细胞毒理学(cytotoxicology) 一、细胞培养 将离体细胞在体外(in vitro)模拟体内环境维持其代谢与生存,进行传代与培养称为细胞培养(cell culture)。在细胞毒理研究中可用原代、二倍体细胞及生物学特性明确的细胞株等。 (一)离体细胞于体外存活的适宜条件 1.温度:最适宜的温度为37(36～38)℃。 2.气体与氢离子浓度:O_2可供给细胞呼吸,CO_2为代谢产物并维持培养液的缓冲能力。适于细胞生存的pH为7.2～7.4。
Since the establishment of cell culture technology by Harrison in 1906, it has been widely used in various fields. Especially in recent years, its application in toxicology has drawn more and more attention. It has gradually formed a new discipline - cytotoxicology, First, cell culture In vitro cells in vitro (in vitro) to mimic the in vivo environment to maintain its metabolism and survival, for passage and culture is called cell culture (cell culture). Cytotoxicity studies available in primary, diploid cells and biological characteristics of clear cell lines. (A) ex vivo cells in vitro conditions suitable for survival 1. Temperature: The most suitable temperature is 37 (36 ~ 38) ℃. 2. Gas and hydrogen ion concentration: O2 can supply cells to breathe, CO2 is the metabolite and maintain the buffer capacity of the culture fluid. The pH suitable for cell survival is 7.2 to 7.4.