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在研究药物抑菌作用中,常采用纸片法、牛津小杯法、平板小沟法和试管稀释法(包括固体和液体培养基)等多种手段,诸方法都各自适合不同对象抑菌作用的研究,且具有简便、快速的优点。然而,测定一些血清制剂或对仅有轻微抑菌作用的物质时,采纳上述方法,却很难准确地表达其抑菌效应,为此,作者试以猪丙种球蛋白(以下简称猪丙球)的抑菌试验为例,将数据经统计学方法处理,发现其结果较常用的几种抑菌试验方法更为准确、可靠,现将该方法介绍如下。一、材料与方法: 1.菌液制备:将白色葡萄球菌和痢疾杆菌(F_2a)6小时培养物,分别稀释成1000
In the study of antibacterial activity of drugs, often using the paper method, the Oxford small cup method, the plate groove method and tube dilution method (including solid and liquid medium) and other means, the methods are suitable for different objects inhibitory effect Research, and has the advantages of simple, fast. However, the determination of some serum preparations or substances with only a slight antibacterial effect, the adoption of the above method, it is difficult to accurately express its antimicrobial effect, for which the author try to porcine gamma globulin (hereinafter referred to as pigs) Antibacterial test, for example, the data were statistically processed and found that the results of several antibacterial test methods more commonly used more accurate and reliable, the method described below. First, the material and methods: 1. Bacterial liquid preparation: The Staphylococcus aureus and Shigella (F_2a) 6 hours culture were diluted to 1000