论文部分内容阅读
目的研究大肠癌组织胃泌素和胃泌素ｍＲＮＡ的表达水平，探讨胃泌素在大肠癌生长中的作用。方法应用放射免疫法检测４０例大肠癌组织和相应的癌旁正常粘膜组织胃泌素，同时应用半定量ＲＴ－ＰＣＲ方法对胃泌素ｍＲＮＡ表达水平进行分析。结果大肠癌组织胃泌素、胃泌素ｍＲＮＡ表达水平明显高于正常对照组（Ｐ＜０．０５），同时发现胃泌素、胃泌素ｍＲＮＡ表达水平和大肠癌的Ｄｕｋｅｓ分期及转移成显著正相关。结论证实胃泌素具有自分泌促肿瘤生长作用，且胃泌素表达的增加很可能发生在肿瘤中晚期，可作为大肠癌预后的重要指标。
Objective To investigate the expression of gastrin and gastrin mRNA in colorectal cancer tissue and to investigate the role of gastrin in the growth of colorectal cancer. Methods Gastrin was detected by radioimmunoassay in 40 cases of colorectal cancer and corresponding normal tissue adjacent to the tumor. Gastrin mRNA expression was analyzed by semi-quantitative RT-PCR. Results The expression of gastrin and gastrin mRNA in colorectal cancer tissue was significantly higher than that in normal control group (P<0.05). At the same time, it was found that gastrin and gastrin mRNA expression levels and Dukes staging and metastasis of colorectal cancer were significantly different. Positively related. Conclusions Gastrin has the function of autocrine promoting tumor growth, and the increase of gastrin expression is likely to occur in the late stage of tumor, which can be used as an important indicator of the prognosis of colorectal cancer.