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应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。利用LDH释放法，观察顺氨氯铂（CDDP）和阿霉素（ADM）预处理人肝癌细胞系H－7402，对CD3AK杀伤该靶细胞的调节作用。ABC－ELISA法检测CDDP和ADM对H-7402细胞表达细胞间粘附分子-1（ICAM-1）、HLA－ABC、HLA－DR抗原的影响。结果表明：经CDDP（2．0μg／ml）、ADM（O．1μg／ml）预处理12h的H－7402细胞对CD3AK的杀伤敏感性显著提高（P＜0.05）；CDDP能够明显促进H－7402细胞表达ICAM-1、HLA－ABC分子（P＜0.05）；ADM预处理的肿瘤细胞表面三种分子的表达均未发生明显的变化。CDDP促进人肝癌细胞对CD3AK的杀伤敏感性可能与其上调了肿瘤细胞表面的ICAM-1分子有关。
Human peripheral blood mononuclear cells were induced by anti-CD3 monoclonal antibody and recombinant human IL-2 to prepare anti-CD3 monoclonal antibody-activated killer cells (CD3AK). Using LDH release method, the human hepatocellular carcinoma cell line H-7402 pretreated with cisplatin (CDDP) and adriamycin (ADM) was observed to modulate CD3AK killing the target cell. The effect of CDDP and ADM on the expression of intercellular adhesion molecule-1 (ICAM-1), HLA-ABC and HLA-DR antigens in H-7402 cells was detected by ABC-ELISA. The results showed that the cytotoxicity of H-7402 cells pretreated with CDDP (2.0μg/ml) and ADM (O.1μg/ml) for CD3AK was significantly increased (P<0.05); CDDP could significantly promote H-7402 The cells expressed ICAM-1 and HLA-ABC molecules (P<0.05). The expression of three molecules on the surface of tumor cells treated with ADM did not change significantly. CDDP promotes the killing sensitivity of human hepatoma cells to CD3AK, which may be related to the upregulation of ICAM-1 molecules on the surface of tumor cells.