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数种抗肿瘤化疗药物具有细胞基因效应,它们不仅诱发细菌和培养的哺乳类细胞突变,而且可以致使实验动物发生癌。最近有报告称,儿童经化疗后的许多年内都存在有染色体的畸变。本文则介绍在丝裂霉素治疗的不同时内肿瘤病人周围淋巴细胞中姊妹染色单体交换(SLE)。从接受丝裂霉素治疗的肿瘤病人中收集的周围淋巴细胞来检查细胞遗传的效应。病人静脉注射丝裂霉素,每周2次,每次4 mg,共2周。体外培养淋巴细胞72小时,培养基含有植物血凝素(P.H.A)和5溴脱氧尿核苷,然后记录姊妹染色单体(SCE)交换。丝裂霉素治疗前,姊妹染色交换的发生率,肿瘤病人与
Several antitumor chemotherapeutic drugs have cellular gene effects that not only induce mutations in bacteria and cultured mammalian cells, but also cause cancer in experimental animals. Recently, there have been reports of chromosomal aberrations in children many years after chemotherapy. This article describes sister chromatid exchange (SLE) in peripheral lymphocytes of tumor patients at different times during mitomycin treatment. Peripheral lymphocytes collected from mitomycin-treated tumor patients were examined for cytogenetic effects. The patient intravenously injected mitomycin 2 times a week for 4 weeks for 2 weeks. Lymphocytes were cultured in vitro for 72 hours. The medium contained phytohemagglutinin (P.H.A.) and 5 bromodeoxyuridine and then the sister chromatid (SCE) exchange was recorded. Prior to mitomycin treatment, the incidence of sister-dye-exchange was associated with cancer patients.