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目的针对珠海口岸分离的霍乱弧菌进行分子分型研究。方法选择46株菌株(包括45株霍乱弧菌和1株弧菌属细菌),采用肠杆菌基因间重复一致序列—聚合酶链反应技术(Enterobacteial Repetitive Intergenic Consensuspolymerase chain reaction,ERIC-PCR)扩增细菌的基因组DNA,对产生的指纹图谱进行聚类分析。结果 ERIC-PCR绘制的基因组指纹图条带清晰可辨且有一定的多态性,聚类分析结果显示ERIC-PCR将46株菌株分为22个聚类群,其中从珠海口岸供澳水产品中分离的30株O1群霍乱弧菌非流行株被细分成12个聚类群。ERIC-PCR能有效区分O1群、O139群和非O1/非O139群霍乱弧菌血清群,也能完全区分霍乱弧菌流行株与非流行株。结论 ERIC-PCR是一种有效的、具有高识别力的霍乱弧菌分型方法。本研究获得的霍乱弧菌分子分型数据为珠海口岸霍乱的防控提供了有价值的基础资料。
Objective To study the molecular typing of Vibrio cholera isolated in Zhuhai Port. Methods 46 strains of bacteria (including 45 strains of Vibrio cholerae and 1 strain of Vibrio) were selected and the bacteria were amplified by Enterobacterial Repetitive Intergenic Consensus Polymerase chain reaction (ERIC-PCR) Of genomic DNA, the fingerprint generated by cluster analysis. Results The bands of genomic fingerprint drawn by ERIC-PCR were clearly identifiable and some polymorphism. Cluster analysis showed that 46 isolates were divided into 22 clusters by ERIC-PCR, Thirty non-epidemic strains of Vibrio cholerae O1 isolates were subdivided into 12 clusters. ERIC-PCR can effectively distinguish between O1 group, O139 group and non-O1 / non-O139 group Vibrio cholera serogroup, but also can completely distinguish between Vibrio cholerae epidemic and non-epidemic strains. Conclusion ERIC-PCR is an effective and highly discriminating method for typing Vibrio cholerae. The Vibrio cholerae molecular typing data obtained in this study provided valuable basic data for the prevention and control of cholera in Zhuhai port.