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目的:探讨常用药对羌活与独活及其单味药治疗佐剂关节炎的作用,为临床羌活、独活配伍用药方式提供实验依据。方法:建立完全弗氏佐剂关节炎模型;造模成功后随机分空白组、模型组、阳性组、羌活组、独活组、羌独活组;观察大鼠机械疼痛阈值、足趾容积变化;酶联免疫吸附试验检测足趾组织研磨液IL-1β、IL-6、TNF-α、PGE2水平。结果:给药第14天后,给药组与模型组相较,大鼠对机械压力刺激的痛阈值显著增大(P<0.05),大鼠的足趾容积显著减小(P<0.05),各给药组均能够显著抑制IL-6、TNF-α、PGE2的水平(P<0.05),但是,羌活、独活组对IL-1β水平无影响,羌独活组能够显著减少IL-1β的产生(P<0.05)。结论:药对羌活与独活及其单味药能够在不同程度上显著性的减轻关节炎大鼠的足趾肿胀,提高机械压力刺激的痛阈值,其抑制IL-1β、IL-6、TNF-α、PGE2的异常分泌可能是治疗佐剂关节炎的机制之一。
Objective: To investigate the effect of commonly used drugs on the adjuvant arthritis of Rhizoma et Radix Notopiae and Rhizoma et Radix Notoginseng and its single herb, and to provide experimental evidence for the clinical use of Rhizoma et Rhizoma. Methods: Complete Freund's adjuvant arthritis model was established. After successful modeling, rats were randomly divided into blank group, model group, positive group, Notopterygium group, Daihuo group and Qiangdu activity group. Changes of mechanical pain threshold and toe volume were observed. The levels of IL-1β, IL-6, TNF-α and PGE2 in the toe tissue abractive fluid were detected by the ELISA. Results: Compared with the model group, the pain threshold of the rats in the mechanical stimulation group was significantly increased (P <0.05) and the volume of the toe in the treated group was significantly reduced (P <0.05) However, Qianghuo and Jiu-active group had no effect on the level of IL-1β, and Qiangduo-live group could significantly reduce the production of IL-1β (P <0.05). CONCLUSION: Rhizoma et Radix et Rhizoma et Radix et Rhizoma Rhei and its single herb can relieve the toe swelling of arthritic rats to varying degrees, increase the pain threshold of mechanical stress stimulation and inhibit the expression of IL-1β, IL-6 and TNF- Abnormal secretion of α and PGE2 may be one of the mechanisms of adjuvant arthritis.