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目的：探讨Ｆａｓ信号通路在肺泡Ⅱ型细胞损伤作用的可能机制及意义。方法：建立大鼠肺泡Ⅱ型细胞体外培养，采用流式细胞术，原位杂交、逆转录－多聚酶链式反应等方法，观察大肠杆菌内毒素（ＬＰＳ）对肺泡Ⅱ型细胞ＦａｓｍＲＮＡ、蛋白质及细胞凋亡的影响。结果：成功建立大鼠肺泡Ⅱ型细胞体外培养技术，ＬＰＳ刺激后，肺泡Ⅱ型细胞ＦａｓｍＲＮＡ、蛋白质表达均显著增加（Ｐ＜０．０１），ＬＰＳ可诱导肺泡Ⅱ型细胞凋亡，并在一定范围内呈量－效关系。结论：ＬＰＳ对肺泡上皮细胞的损伤效应，除直接损伤作用外，还可能通过诱导Ｆａｓ抗原表达增加，而致细胞凋亡的方式进行
Objective: To investigate the possible mechanism and significance of Fas signaling pathway in alveolar type 2 cell injury. Methods: The alveolar type Ⅱ cells were cultured in vitro. The effects of E. coli lipopolysaccharide (LPS) on the expression of Fas mRNA, protein and cell in alveolar type Ⅱ cells were observed by flow cytometry, in situ hybridization and reverse transcription polymerase chain reaction Effect of apoptosis. RESULTS: The alveolar type 2 cells were successfully established in vitro. After LPS stimulation, the expression of Fas mRNA and protein in alveolar type 2 cells increased significantly (P <0.01). LPS induced alveolar type 2 cell apoptosis, Within the range of quantitative - effective relationship. Conclusion: The injury effect of LPS on alveolar epithelial cells, in addition to direct injury, may also induce apoptosis by increasing the expression of Fas antigen