用双核淋巴细胞微核试验来检测接触射线人群的细胞遗传效应

• 作 者: 何继亮 金海燕 金力奋 高慎永
• 机 构: 浙江医科大学环境卫生教研室
• 出 处: 中国公共卫生
• 发表日期: 1998年03期
• 关键词: 微核 染色体畸变 微核试验 细胞遗传 微核细胞率 筛检 检测指标 畸变率 染色体损伤 体外实验研究

采用双核细胞微核试验（ＣＢＭＮ）对放射病人组、接触射线组和对照组外周血ＭＮ进行检测，ＭＮ率分别为１３２３％，４２０％和３２６‰。放射病人组明显高于其他两组（Ｐ＜００１）。接触射线组与对照组之间差异不显著（Ｐ＞００５）。放射病人组中异常ＭＮ率人数高达９０％。本实验还对３组人员的ＣＡ作了检测，结果与ＭＮ相似，分别为２０６％，０９３％和０６９％，放射病人组明显高于其他两组（Ｐ＜００５和Ｐ＜００１）。接触射线组与对照组之间差异不显著（Ｐ＜００５）。实验表明ＣＢＭＮ方法快速、灵敏、准确，适用于大样本人群辐射遗传损伤的筛检。

The peripheral blood MN in radiotherapy group, exposure radiography group and control group were detected by using binucleated cell micronucleus test (MNMN), with MN rates of 1323%, 420% and 326 ‰, respectively. The radiotherapy group was significantly higher than the other two groups (P <001). There was no significant difference between the contact ray group and the control group (P> 005). Radiation patients in the group of abnormal MN rate as high as 90%. The experiment also detected the CA of 3 groups of patients with similar results to MN, which were 206%, 093% and 069%, respectively. The radiotherapy group was significantly higher than the other two groups (P <005 And P <001). There was no significant difference between the contact ray group and the control group (P <005). Experiments show that CBMN method is rapid, sensitive and accurate and suitable for the screening of genetic damage of radiation in a large sample population.