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采用花粉发育时期为单核至双核初期的白菜(Brassica Pekinensis Rupr.)花药进行离体培养。诱导产生愈伤组织的培养基为:B_5、MS,附加不同浓度的2,4-D、NAA、KT(或6BAP)、腺嘌呤,温度为32～24℃。诱导愈伤组织分化的培养基为:B_5(或MS)+6BAP 2毫克/升+蔗糖1.5％,温度为24～17℃。生根培养基为White+NAA 0.05毫克/升+IAA 0.2毫克/升+蔗糖1.5％。已在多种品种(组合)上获得较大量的花粉植株,为进一步开展单倍体育种提供了试材。 小植株根尖、幼叶体细胞染色体镜检n=10,为单倍体植株。
In vitro culture of Brassica Pekinensis Rupr. Anther with mononuclear to early dinuclear stage was carried out. The medium that induced callus was: B_5, MS, with different concentrations of 2,4-D, NAA, KT (or 6BAP), adenine, and temperature of 32-24 ° C. The medium for inducing callus differentiation was: B_5 (or MS) + 6BAP 2 mg / L + 1.5% sucrose at a temperature of 24-17 ° C. The rooting medium was White + NAA 0.05 mg / L + IAA 0.2 mg / L + Sucrose 1.5%. A large number of pollen plants have been obtained on a variety of varieties (combinations), providing a test material for further haploid breeding. Plantlets apical, juvenile somatic cell chromosome microscopy n = 10, haploid plants.