论文部分内容阅读
目前大量制备均一性抗体的方法主要有杂交瘤细胞的体外培养及动物腹水两种。本文作者报告了另外一种更新更吸引人的方法,这就是用植物制备抗体。用植物制备抗体的关键是构建转基因植物。他们的研究系统以烟草细胞作表达宿主,制备一种具有催化功能的抗体;以观察植物细胞是否能够加工并装配功能性免疫球蛋白。具体步骤是:首先将重链及轻链的cDNA 插入一种土壤菌,再用其分别转染烟草细胞。这样产生的烟草便可表达重链或轻
At present, a large number of methods for preparing uniform antibodies mainly include in vitro culture of hybridoma cells and animal ascites. The authors of the report reported another, more intriguing, approach to making antibodies that are made from plants. The key to making antibodies from plants is to build transgenic plants. Their research system uses tobacco cells as a host of expression to prepare a catalytic antibody; to see if plant cells can process and assemble functional immunoglobulins. Specific steps are: First, the heavy chain and light chain cDNA inserted into a soil bacteria, and then were transfected into tobacco cells. The tobacco thus produced can express heavy chains or light