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从玉米重病田病苗上，分离到了Ｒｈｉｚｏｃｔｏｎｉａｓｏｌａｎｉ，Ｆｕｓａｒｉｒｍｓｏｌａｎｉ和Ｐｙｔｈｉｕｍｓｐｐ．三种致病菌。前二种病菌的分离率较高，Ｒｈｉｚｏｃｔｏｎｉａｓｏｌａｎｉ的致病力最强，为玉米苗期根腐病的主要病原菌。主要致病菌的侵染适期为玉米苗龄１０～１５ｄ，田间采样适期在苗龄为１５～２５ｄ内。不同种类的表面处理剂对Ｒｈｉｚｏｃ－ｔｏｎｉａｓｏｌａｎｉ的分离率有一定的影响，建议用无菌水做玉米苗期根腐病组织分离的表面处理剂。低温有利于病菌的侵染。
Rhizoctonia solani, Fusarirmsolani and Pythiumspp were isolated from the diseased corn field. Three kinds of pathogens. The first two kinds of bacteria separation rate is higher, Rhizoctoniasolani strongest pathogenicity, the main pathogen of corn root rot. The main pathogen infection period of maize seedling age 10 ~ 15d, field sampling in the appropriate age of 15 ~ 25d. Different types of surface treatment agents have an impact on the isolation rate of Rhizoctonia tonni. It is recommended to use sterile water as the surface treatment agent to separate the root rot tissue of corn seedlings. Low temperature is conducive to the infection of germs.