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利用原代细胞培养技术，观察了不同浓度的铅（Ｐｂ）对支持细胞（Ｓｅｒｔｏｌｉ）功能的影响。结果发现，Ｐｂ２＋（１０－７～１０－４ｍｏｌ／Ｌ）使处理２４ｈ的Ｓｅｒｔｏｌｉ细胞乳酸含量显著升高；Ｐｂ２＋（１０－５～１０－４ｍｏｌ／Ｌ）也使丙酮酸含量明显升高。Ｐｂ２＋（１０－５ｍｏｌ／Ｌ）使处理６ｈ和２４ｈ的Ｓｅｒｔｏｌｉ细胞乳酸含量分别显著低于和高于对照水平；Ｐｂ２＋（１０－７～１０－４ｍｏｌ／Ｌ）使处理２４ｈ的Ｓｅｒｔｏｌｉ细胞内ｃＡＭＰ含量显著下降。醋酸锌（Ｚｎ２＋）对培养的Ｓｅｒｔｏｌｉ细胞的上述观察指标均无影响。因此，用这些指标的变化可以评价Ｐｂ２＋对睾丸Ｓｅｒｔｏｌｉ细胞的某些毒性。
Using primary cell culture techniques, the effect of different concentrations of lead (Pb) on the function of Sertoli cells was observed. The results showed that Pb2 + (10-7 ~ 10-4mol / L) significantly increased the content of lactic acid in Sertoli cells treated with Pb2 + (10-5 ~ 10-4mol / L). Pb2 + (10-5mol / L) significantly decreased the lactic acid content of Sertoli cells treated for 6h and 24h, respectively. The level of cAMP in Sertoli cells treated with Pb2 + (10-7-10-4mol / L) for 24h was significantly higher than that of control decline. Zinc acetate (Zn2 +) had no effect on the above observations of cultured Sertoli cells. Therefore, some of the toxicity of Pb2 + to testicular Sertoli cells can be assessed using changes in these indicators.