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目的:对比研究鼻咽癌和鼻息肉标本中VEGF表达强度及MVD差异,同时分析VEGF、MVD和鼻咽癌临床特征的相关性。方法:纳入我科就诊的鼻咽癌患者57例,鼻息肉患者50例。采用免疫组化SABC法检测癌组织、癌旁组织、及息肉组织中中VEGF蛋白的表达,及MVD强度。分析VEGF、MVD和鼻咽癌患者性别、临床分期、颈部淋巴结转移、远处转移、血清EBV-Ig A阳性、WHO病理分型相关性。统计分析随访结果,对可能影响鼻咽癌预后的因素进行Cox回归模型分析。结果:鼻咽癌组织、鼻咽癌旁组织、鼻息肉组织中VEGF表达、MVD强度具有明显差异(p<0.05)。不同鼻咽癌临床分期、是否发生远处转移、不同WHO病理分型和VEGF表达、MVD强度具有明显差异(p<0.05)。Cox回归方程显示,远处转移、病理分型、VEGF表达强度是影响鼻咽癌生存的独立危险因素(p<0.05)。结论:鼻咽癌高表达VEGF,促进新生血管,形成高密度微小血管,和鼻咽癌远处转移密切相关,降低其生存率。
OBJECTIVE: To compare the differences of VEGF expression and MVD in nasopharyngeal carcinoma and nasal polyps specimens, and to analyze the correlation between the clinical features of VEGF, MVD and nasopharyngeal carcinoma. Methods: 57 patients with nasopharyngeal carcinoma and 50 patients with nasal polyp included in our department were enrolled. Immunohistochemical SABC method was used to detect the expression of VEGF protein and MVD in cancer tissues, adjacent tissues and polyps tissues. The correlation of VEGF, MVD and nasopharyngeal carcinoma patients’ gender, clinical stage, cervical lymph node metastasis, distant metastasis, serum EBV-Ig A positive and WHO pathological classification were analyzed. Statistical analysis of follow-up results, factors that may affect the prognosis of nasopharyngeal carcinoma Cox regression model analysis. Results: The expression of VEGF and MVD in nasopharyngeal carcinoma, nasopharyngeal carcinoma and nasal polyps were significantly different (p <0.05). There were significant differences in MVD intensity between different NPC stages, distant metastasis, different WHO pathological types and VEGF expression (p <0.05). Cox regression equation showed that distant metastasis, pathological type and VEGF expression were independent risk factors for the survival of NPC (p <0.05). Conclusion: High expression of VEGF in nasopharyngeal carcinoma promotes neovascularization and formation of high-density microvascular and is closely related to the distant metastasis of nasopharyngeal carcinoma, reducing its survival rate.