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微丝蚴分离技术,1974年以来国外已陆续报告,八十年代进展甚速。我们参照国外经验做了改进,分离出纯净活的微丝蚴,现将几种分离方法报告如下。材料和方法一、聚蔗糖梯度离心分离法:从长爪沙鼠腹腔液中分离微丝蚴。 (一)聚庶糖[Ficoll,分子量400,000Type400(sigma)]以生理盐水成PBS配制10％浓度的溶液; (二)取5ml10％Ficoll溶液置10ml锥
Microfilaria separation technology, since 1974 has been reported abroad, the rapid progress of the eighties. We have made reference to improve the experience of foreign countries, the separation of pure living microfilaria, now several methods of separation are reported as follows. Materials and methods First, the separation of ficoll gradient centrifugation: isolated from the Meriones unguiculatus microfilariae. (A) poly sucrose [Ficoll, molecular weight 400,000Type400 (sigma)] to normal saline into PBS to prepare a 10% concentration solution; (B) take 5ml10% Ficoll solution set 10ml cone