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本文论述了识别肥大细胞亚型及其前体细胞的一种实验手段。将纯度为95%～99%的大鼠腹膜肥大细胞裂解,制成细胞膜非颗粒性抗原,经腹膜免疫小鼠,同时把提纯的完整的肥大细胞注入免疫小鼠的腋淋巴结中。随后,将免疫小鼠的脾细胞与P_3ol 骨髓瘤细胞融合。通过间接免疫荧光法可检测出这种杂交瘤产生了一种抗体,即抗大鼠肥大细胞单克隆抗体(RMC-McAb)。该抗体所作用的靶细胞包括大鼠颗粒细胞、腹膜渗出的单核细胞、巨噬细胞、胸腺细胞和骨髓细
This article discusses an experimental means of identifying mast cell subtypes and their precursors. The rat peritoneal mast cells were lysed with purity of 95% -99% to prepare the non-granular antigen of the cell membrane. The mice were peritoneally immunized, and the purified intact mast cells were injected into the axillary lymph nodes of the immunized mice. Subsequently, the splenocytes of immunized mice were fused with P3 mol myeloma cells. This hybridoma can be detected by indirect immunofluorescence to produce an antibody, anti-rat mast cell monoclonal antibody (RMC-McAb). The target cells to which the antibody acts include rat granulosa cells, peritoneal exudate monocytes, macrophages, thymocytes and bone marrow fine