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为了弥补原核生物短期试验揭示诱变抑制剂之不足,建立了体外培养哺乳类V_(79)细胞微核抑制试验检测诱变抑制剂,并对该试验作了区分去诱变剂和抗诱变剂设计,辅以受试物对细胞生长影响,以揭示真正的诱变抑制剂。实验证明,大蒜素0.06mg/L、0.6mg/L能降低MNNG 0.1mg/L和环磷酰胺2mg/L诱发V_(79)细胞微核形成率(P<0.05),但它对细胞增殖亦有抑制作用。白术水提取液1.25×10~3mg/L、5×10~3mg/L在代谢活化系统存在情况下,可抑制环磷酰胺2mg/L诱发V_(79)细胞微核的形成(P<0.05),推测它可能对代谢活化酶系有揶制作用,有待进一步实验证实。
In order to make up for the lack of mutagenesis inhibitors in short-term trials of prokaryotic organisms, a mutagenic inhibitor was established in a micronucleus suppression assay of mammalian V_(79) cells in vitro, and a differential mutagenic agent and antimutagenesis was established for this test. The agent design, supplemented with the effects of the test substance on cell growth, reveals the true mutagenesis inhibitor. Experiments showed that allicin 0.06mg/L, 0.6mg/L can reduce the micronucleus formation rate of V_(79) cells induced by MNNG 0.1mg/L and 2mg/L of cyclophosphamide (P<0.05), but it also has an effect on cell proliferation. There is inhibition. Atractylodes macrocephala aqueous extract 1.25×10~3mg/L and 5×10~3mg/L could inhibit the formation of micronuclei in V_(79) cells induced by cyclophosphamide 2mg/L in the presence of metabolic activation system (P<0.05). , It is speculated that it may be used for the production of metabolically active enzyme systems, and further experiments are needed to confirm it.