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作者比较了常见环境污染物——金属可溶性盐类——对细胞的毒性。用冲洗法洗出兔肺泡巨噬细胞,其量为2.2×10~6个/毫升。将不同浓度(1～500微克/毫升)的Cd~(2+)、Ni~(2+)、Mn~(2+)、Cr~(3+)的氯化物和VO_3~-(钒酸铵)分别加入组织培养液中,在37℃下培养20小时。然后用计数池直接进行细胞计数;用光学显微镜观察至少200个细胞的活力;用0.1M醋酸缓冲液在pH5.0时测定酸性磷酸酶的比活性;以及用扫描电子显微镜观察细胞表面形态。以NaCl作对照,Na浓度高达1.000微克/毫升。
The authors compared the common environmental pollutants - metal soluble salts - on cell toxicity. Flushing out of rabbit alveolar macrophages, the amount of 2.2 × 10 ~ 6 / ml. Cd 2+, Ni 2+, Mn 2+ and Cr 3+ chlorides and VO 3 - (ammonium vanadate) solutions with different concentrations (1 ~ 500 μg / ml) ) Were added to the tissue culture medium and incubated at 37 ° C for 20 hours. The cell counts were then counted directly with a counting cell; the viability of at least 200 cells was observed with an optical microscope; the specific activity of acid phosphatase was measured with 0.1 M acetate buffer at pH 5.0; and the cell surface morphology was observed with a scanning electron microscope. NaCl as a control, Na concentration up to 1.000 micrograms / ml.