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研究结果表明，利用热研二号柱花草无菌苗的子叶为外植体，在M2培养基（MS基本成分＋0．8％琼脂＋3．0％蔗糖＋1．0mg／L萘乙酸＋4．0mg／L激动素，pH6．0）培养可诱导分化出愈伤组织和枝条。1月龄枝条在M7培养基（1／2MS基本成分＋0．8％琼脂＋1．0％蔗糖＋0．5mg／L萘乙酸＋0．05％活性炭，pH6．0）培养可诱导生根，生根率60％，获得了具有根、茎、叶的完整柱花草组培植株。25和50μg／ml浓度的卡那霉素均能完全抑制子叶形成愈伤组织和愈伤组织再分化。
The results showed that the cotyledons of hot germ-free plantlets were used as explants in M2 medium (MS basic + 0.8% agar + 3.0% sucrose + 1.0 mg / L NAA + 4.0 mg / L Kinetin, pH 6.0) can induce differentiation of callus and shoots. One-month-old shoots could induce rooting in M7 culture medium (1 / 2MS basic composition + 0.8% agar + 1.0% sucrose + 0.5mg / L NAA + 0.05% activated carbon, pH6.0) , Obtained a complete plant with root, stem and leaf tissue culture. Both kanamycin at 25 and 50 μg / ml completely inhibited cotyledon callus and callus redifferentiation.