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在硬粒小麦－簇毛麦双二倍体（ＴＨ３）／４×Ｗａｎ７１０７ 的幼胚培养及花药培养后代中选育出普通小麦－簇毛麦抗白粉病易位系Ｐｍ ９７０３３ 等。利用白粉病抗性鉴定、细胞遗传学分析、生化标记、分子原位杂交等手段，鉴定出该材料为６ＤＬ／６ＶＳ臂间易位系。其根尖细胞染色体数为４２，花粉母细胞减数分裂中期Ⅰ染色体构型为２１Ⅱ。它与农艺亲本Ｗａｎ７１０７ 及６Ｄ／６Ｖ 异代换系９６Ｎ６２１－３－７－３ 测交Ｆ１ 的ＰＭＣｓＭⅠ染色体构型均为２１Ⅱ，频率分别为９２．５％ 和７９．４％ 。与中国春６Ａ、６Ｂ、６Ｄ 重双端体测交Ｆ１ 的ＰＭＣｓＭⅠ染色体构型分别为２１Ⅱ＋ １ 个异型二价体（ｔⅠｔ），２１Ⅱ＋ １ 个异型二价体（ｔⅠｔ）及２０Ⅱ＋ １ 个异型二价体（Ⅰｔ）＋ １ 个端体（ｔ），出现频率分别为８９．４％ 、８５．４％ 和９４．３％ 。生化分析结果表明，它缺失簇毛麦６ＶＬ上的谷草转氨酶ＧＯＴ－Ｖ２ 位点，而具有６ＶＳ上的醇溶蛋白Ｇｌｉ－Ｖ２ 位点。分子原位杂交结果表明， Ｐｍ ９７０３３、Ｐｍ ９７０３４和Ｐｍ ９７０３５ 均为纯合的臂间易位系。易位系及其测交Ｆ１ 均对白粉病表现免疫
The common wheat-cluster-bearing wheat powdery mildew resistant translocation line Pm 97033 was bred in the immature embryo culture and anther culture progeny of durum wheat-cluster bmer diploid (TH3) / 4 × Wan7107. This material was identified as a 6DL / 6VS arm-arm translocation line using powdery mildew resistance assays, cytogenetic analysis, biochemical markers, and molecular in situ hybridization. The number of chromosomes in root tip cells was 42 and that in metaphase Ⅰ of pollen mother cells was 21 Ⅱ. The PMCsM1 chromosomal structure of F1 hybrids was found to be 21II with frequencies of 92.5% and 79.4%, respectively, in F1 hybrids of Wan7107 and 6D / 6V line 96N621-3-7-3. The chromosomal patterns of PMCsMⅠof F1 hybrids with 6A, 6B and 6D in Chinese spring were 21Ⅱ + 1 heterotrophic (tⅠt), 21Ⅱ + 1 heterotrophic (tⅠt) and 20Ⅱ + 1 heterotrophic (Ⅰt) + 1 terminal (t), the frequency of occurrence was 89.4%, 85.4% and 94.3% respectively. The results of biochemical analysis showed that it was deficient in the GOT-V2 site of aspartate aminotransferase on 6VL and had the Gli-V2 site of 6VS. Molecular in situ hybridization results showed that Pm 97033, Pm 97034 and Pm 97035 were homozygous translocations. The translocation lines and their F1 cross were all immunized against powdery mildew