论文部分内容阅读
目的 探讨新生儿肺炎白细胞介素 (IL) 10、 13与免疫球蛋白 (Ig)的关系。 方法 采用免疫酶法(ELISA)和速率散射比浊法检测新生儿肺炎患儿血IL 10、IL 13、IgG、IgA、IgM。以C反应蛋白 (CRP)≥ 2 0mg/L作为诊断细菌感染的界限值 ,结合临床资料 ,将肺炎分为 4组进行结果分析。结果 1.肺炎组 8型常见病毒及支原体特异性IgM阳性 4 0份 (36 .0 % ) ;对照组 30份血清检测均阴性。病毒及支原体感染 (病毒感染 ) 2 3例(2 0 .7% ) ,细菌感染 4 5例 (4 0 .5 % ) ,混合感染 17例 (15 .3% ) ,不明病原感染 (其他感染 ) 2 6例 (2 3.4 % )。 2 .肺炎组IgA、IgM明显高于对照组 (P <0 .0 5 )。其中病毒感染组IgA明显高于其他感染组和对照组 (P <0 .0 5 ) ;IgM含量为细菌感染组显著高于对照组 (P <0 .0 5 )。 3.病毒感染组IgG、IgA、IgM分别与IL 10有显著相关 (P<0 .0 5 )。混合感染组、细菌感染组和对照组IgM分别与其IL 13呈显著相关 (P <0 .0 5 )。结论 新生儿肺炎时 ,IgA是完成抗病毒体液免疫应答的重要成分 ,IL 10对IgA产生具有调节作用 ;IgM能在抗菌性体液免疫机制中发挥重要作用 ,IL 13有助于调节IgM产生
Objective To investigate the relationship between neonatal pneumonia interleukin (IL) 10,13 and immunoglobulin (Ig). Methods The levels of IL 10, IL 13, IgG, IgA and IgM in children with neonatal pneumonia were measured by enzyme-linked immunosorbent assay (ELISA) and rate nephelometry. C reactive protein (CRP) ≥ 20mg / L as a diagnostic threshold for bacterial infection, combined with clinical data, the pneumonia is divided into four groups for analysis of the results. Results 1 pneumonia group of 8 common viruses and mycoplasma-specific IgM positive 40 (36.0%); control group of 30 serum tests were negative. There were 23 cases (20.7%) of virus and mycoplasma infection (20.7%), 45 cases (4.0%) of bacterial infection, 17 cases (15.3%) of mixed infection, unknown pathogen infection (other infections) 26 cases (23.4%). The levels of IgA and IgM in pneumonia group were significantly higher than those in control group (P <0.05). The level of IgA in the virus-infected group was significantly higher than that in other infected and control groups (P <0.05). The IgM level in the bacterial infection group was significantly higher than that in the control group (P <0.05). 3. The virus infection group IgG, IgA, IgM and IL 10 were significantly correlated (P <0.05). IgM in mixed infection group, bacterial infection group and control group were significantly correlated with IL13 (P <0.05). Conclusions IgA is an important component of anti-virus humoral immune response in neonatal pneumonia. IL 10 may play an important role in IgA production. IgM may play an important role in anti-bacterial humoral immune response. IL 13 may help regulate IgM production