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Aim:To determine whether cysteinyl leukotriene receptor 1(CysLT_1 receptor)isinvolved in N-methyl-D-aspartate(NMDA)-induced excitotoxic injury in the mousebrain.Methods:Brain injury was induced by NMDA microinjection(50-150 nmolin 0.5 μL)into the cerebral cortex.The changes in CysLT_1 receptor expression24 h after NMDA injection and the effects of a CysLT_1 receptor antagonist,pranlukast(0.01 and 0.1 mg/kg),an NMDA receptor antagonist,ketamine(30mg/kg),and an antioxidant,edaravone(9 mg/kg)were observed.Results:In theNMDA-injured brain,the CysLT_1 receptor mRNA,and protein expression wereupregulated,and the receptor was mainly localized in the neurons and not in theastrocytes.Pranlukast,ketamine and edaravone decreased NMDA-induced injury;pranlukast(0.1 mg/kg)and ketamine inhibited the upregulated expression of theCysLT_1 receptor.Conclusion:CysLT_1 receptor expression in neurons isupregulated after NMDA injection,and NMDA-induced responses are inhibitedby CysLT_1 receptor antagonists,indicating that the increased CysLT_1 receptor isinvolved in NMDA excitotoxicity.
Aim: To determine whether cysteinyl leukotriene receptor 1 (CysLT_1 receptor) is in released in N-methyl-D-aspartate (NMDA) -induced excitotoxic injury in the mouserain. Methods: Brain injury was induced by NMDA microinjection (50-150 nmolin 0.5 μL) into the cerebral cortex. The changes in CysLT_1 receptor expression24 h after NMDA injection and the effects of a CysLT_1 receptor antagonist, pranlukast (0.01 and 0.1 mg / kg), an NMDA receptor antagonist, ketamine (30 mg / kg) Results: In the NMDA-injured brain, the CysLT_1 receptor mRNA, and protein expression were upregulated, and the receptor was mainly localized in the neurons and not in the astrocytes. Pranlukast, ketamine and edaravone decreased NMDA- induced injury; pranlukast (0.1 mg / kg) and ketamine inhibited the upregulated expression of theCysLT_1 receptor.Conclusion: CysLT_1 receptor expression in neurons is regulated by NMDA injection, and NMDA-induced responses are inhibited by CysLT_1 receptor antagonists, indicating that the increased CysLT_1 receptor isinvolved in NMDA excitotoxicity.