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从黄河三角洲盐碱土壤中分离到一株真菌PSF2,该菌具有极强的溶磷能力,在改良孟金娜液体培养基中培养72 h,发酵液中的可溶性磷含量高达1042.0 mg L-1,且兼具耐盐特性,最高可以耐受12.5%的盐浓度。经形态观察及ITS序列分析,初步确定该菌株为草酸青霉(Penicillium oxalicum),Gen Bank登录号为KJ957935。在单因素试验基础上,采用正交试验设计对菌株PSF2的发酵条件进行了优化,结果表明菌株PSF2的最佳培养基组成是蔗糖15 g L-1、尿素0.14 g L-1、Na Cl 3.0%,p H 9.0。进一步研究表明,在含盐量0.45%的土壤中添加PSF2,培养20 d,土壤中有效磷含量可增加20.9%。
A strain of fungi PSF2 was isolated from saline-alkali soil in the Yellow River Delta. The fungus PSF2 had strong ability of dissolving phosphorus. After cultured for 72 h in modified Mengjinna liquid medium, the soluble phosphorus content in the fermentation broth was as high as 1042.0 mg L-1 Both salt tolerance, up to 12.5% salt concentration. The morphological observation and ITS sequence analysis confirmed that the strain was Penicillium oxalicum, Gen Bank accession number was KJ957935. On the basis of single factor experiment, orthogonal design was used to optimize the fermentation conditions of strain PSF2. The results showed that the optimum culture medium of PSF2 was sucrose 15 g L-1, urea 0.14 g L-1, Na Cl 3.0 %, p H 9.0. Further studies showed that PSF2 was added to 0.45% salinity soil and the content of available phosphorus in soil increased 20.9% after 20 d cultivation.