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目的应用骨生物力学和Micro-CT技术,探讨辅酶Q10对10月龄小鼠股骨的显微结构和生物力学的影响。方法 35只SPF级8月龄雌性KM小鼠,随机分为正常对照组(CON)、维生素E组(VE)、辅酶Q10低剂量组(Co Q10L)、辅酶Q10高剂量组(Co Q10H),辅酶Q10低剂量联合维生素E(Co Q10L+VE),连续灌胃给药10周。实验结束后,取右侧股骨进行Micro-CT扫描及三维重建,左侧股骨进行生物力学检测。结果与CON组比较,Co Q10L组小鼠右侧股骨的微观参数:体积分数、连接密度和骨密度明显增加,左侧股骨的骨生物力学刚度增加。Co Q10H体积分数、连接密度和SMI明显增加,同时骨生物力学参数中弹性载荷和最大位移得到明显增加。Co Q10L联合VE组连接密度增加,生物力学方面参数没有统计学差异。与VE比较,辅酶Q10两个剂量和辅酶Q10低剂量联合维生素E参数并没有统计学差异。结论 Co Q10(500 mg·kg-1·d-1)能够修复小鼠的股骨微观结构,提高骨生物力学的性能,修复骨量、降低股骨骨折风险的能力优于VE,提示Co Q10的抗骨质疏松作用具有良好的研究前景。
Objective To investigate the effects of coenzyme Q10 on the femur microstructure and biomechanics of 10-month-old mice using bone biomechanics and Micro-CT. Methods 35 SPF female KM mice aged 8 months were randomly divided into normal control group (CON), vitamin E group (VE), Co Q10L, Co Q10H, Coenzyme Q10 low-dose combined with vitamin E (Co Q10L + VE), continuous oral administration of 10 weeks. After the experiment, take the right femur for Micro-CT scan and three-dimensional reconstruction, the left femur for biomechanical testing. Results Compared with the CON group, the micro-parameters of right femur in Co Q10L group: the volume fraction, the connection density and the bone density increased obviously, and the biomechanical rigidity of the left femur increased. Co Q10H volume fraction, connection density and SMI increased significantly, while the biomechanical parameters of the elastic load and the maximum displacement was significantly increased. Co Q10L joint VE group increased the connection density, biomechanical parameters there is no statistical difference. Compared with VE, coenzyme Q10 two doses and coenzyme Q10 low dose combination of vitamin E parameters and no statistically significant difference. Conclusions Co Q10 (500 mg · kg-1 · d-1) can repair the femur microstructure and improve the biomechanical properties of the mice. The ability of repairing bone mass and reducing the risk of femoral fracture is better than that of VE, suggesting that Co Q10 Osteoporosis has good research prospects.