论文部分内容阅读
目的了解舰艇官兵执行远航任务前期、中期、后期皮肤细菌的变化特点,为舰艇官兵皮肤病和其他疾病的防治提供依据。方法用无菌采样拭子采集脐周皮肤标本150份,进行细菌培养和分纯,取纯培养细菌进行基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)鉴定。结果从150份脐周皮肤标本分离出病原菌14种47株,主要病原菌有金黄色葡萄球菌、产酸克雷伯菌、阴沟肠杆菌和铜绿假单胞菌;分离出栖居菌11种181株,主要栖居菌有表皮葡萄球菌、人葡萄球菌、溶血葡萄球菌和头状葡萄球菌。任务后期与前期比较,病原菌数增加,栖居菌数减少。医疗组与机电组比较,任务前期、中期、后期脐周病原菌检出数之间差异有统计学意义(P<0.05),栖居菌检出数之间差异无统计学意义(P>0.05)。结论远航期间舰艇官兵容易发生疲劳,免疫功能下降,皮肤正常栖居菌减少,而病原菌增加,容易发生皮肤病及其他疾病。
Objective To understand the characteristics of skin bacteria changes in the early, mid-term and post-mission of naval vessels and their warships, and provide the basis for prevention and treatment of skin diseases and other diseases of naval officers and men. Methods 150 samples of umbilical skin were collected with sterile sampling swabs for bacterial culture and purification. Pure culture bacteria were identified by MALDI-TOF-MS. Results Fourteen 47 strains of pathogenic bacteria were isolated from 150 umbilical skin specimens. The main pathogens were Staphylococcus aureus, Klebsiella oxytoca, Enterobacter cloacae and Pseudomonas aeruginosa. , Staphylococcus aureus, Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus aureus. The latter part of the task compared with the previous period, the number of pathogenic bacteria increased, reducing the number of habitat. There were significant differences in the number of pathogenic bacteria detected in the umbilical cord between the medical group and the mechanical and electrical groups (P <0.05), but there was no significant difference between the detection numbers of the resident bacteria (P> 0.05) . Conclusions During the voyage, naval officers and men are prone to fatigue, the immune function is decreased, the normal skin habitat is reduced, and the number of pathogenic bacteria is increased. Skin diseases and other diseases are prone to occur.