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2013年江苏省高考生物23题考查了温度、pH对酶活性影响实验。试题:为了探究温度、pH对酶活性的影响,下列实验设计不合理的是(表1):答案:ACD该题的D选项引起了很多教师的讨论,有的认为该选项是合理的,有的认为不合理(认为将斐林试剂换成碘液就合理了),他们都没有考虑到pH对斐林试剂和碘液的影响。笔者在实验教学中,重新科学设计了该实验,并收到良好的教学效果(当然,该题的答案也值得商榷)。一、常规实验设计的缺陷按常规设计并完成以下实验(表2):按表1实验设计进行实验,若是用斐林试剂作
In 2013 Jiangsu province college entrance examination biology 23 questions examines the temperature, the pH effect experiment to the enzyme activity. Test questions: In order to explore the effect of temperature and pH on enzyme activity, the following experimental design is unreasonable (Table 1): Answer: ACD D option of this question has aroused many teachers’ discussions, and some think this option is reasonable, there Considered unreasonable (it would be reasonable to replace the Fehling reagent with iodine) and did not consider the effect of pH on the Fehling reagent and iodine solution. In experimental teaching, the author re-designed the experiment scientifically and received good teaching results (of course, the answer to this question is also worth discussing). First, the conventional experimental design defects According to conventional design and complete the following experiments (Table 2): Experimental design according to Table 1 experiments, if the use of Feilin reagents