肝细胞癌患者外周血中miR-26b的表达及意义

• 作 者: 陈浩宇 嘉红云 吴晓蔓
• 机 构: 广州医科大学附属第二医院检验科,
• 出 处: 中华实用诊断与治疗杂志
• 发表日期: 2016年12期
• 关键词: 肝细胞癌 甲胎蛋白 miR-26b fetoprotein 临界值 实时荧光定量 微粒子 化学发光 特异度 曲线分析

目的评估外周血中循环miR-26b表达对肝细胞癌(hepatocellular carcinoma,HCC)的诊断价值。方法 50例HCC患者为观察组,50例体检健康者为对照组,采用实时荧光定量PCR检测2组miR-26b的表达水平;采用化学发光微粒子免疫检测法测定甲胎蛋白(alpha fetoprotein,AFP)水平,应用ROC曲线分析miR-26b联合AFP检测对HCC诊断的临床价值。结果观察组miR-26b表达水平(0.678±0.019)低于对照组(0.996±0.024)(P<0.05);miR-26b水平对诊断HCC的AUC为0.843,临界值为0.890μg/L,诊断敏感度、特异度分别为86%和90%;AFP诊断HCC的AUC为0.756,以常规20.000μg/L作为血清AFP诊断HCC的临界值,其灵敏度、特异度分别为44%和91%;miR-26b联合AFP诊断HCC的敏感度、特异度均为92%。结论 HCC患者外周血miR-26b呈低表达,miR-26b联合AFP检测对诊断HCC具有一定价值。

Objective To evaluate the diagnostic value of circulating miR-26b in peripheral blood for the diagnosis of hepatocellular carcinoma (HCC). Methods Fifty patients with HCC were selected as observation group and 50 healthy controls as control group. The expression of miR-26b in two groups was detected by real-time fluorescence quantitative PCR. The levels of alpha fetoprotein (AFP) Level, ROC curve analysis of miR-26b combined with AFP detection of HCC diagnostic value. Results The miR-26b expression level in observation group (0.678 ± 0.019) was lower than that in control group (0.996 ± 0.024) (P <0.05). The AUC of miR-26b in diagnosis of HCC was 0.843 and the critical value was 0.890μg / L Specificity and specificity were 86% and 90%, respectively. The AUC of AFP was 0.756 and the sensitivity and specificity of serum AFP was 44% and 91% respectively with routine 20.000μg / L as serum AFP. The miR- 26b combined with AFP diagnosis of HCC sensitivity, specificity were 92%. Conclusion The expression of miR-26b in peripheral blood of HCC patients is low, and the combination of miR-26b and AFP is of value in the diagnosis of HCC.