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我们于1979年5月至1980年4月,对住院的285例各期日本血吸虫病患者进行了细胞免疫和体液免疫机能的测定,并与正常人进行比较,同时,观察了急性病例血清蛋白电泳和环卵沉淀反应,对其中部分病例,经有效药物治疗后2周,2个月左右及4~9个月,进行了随访。材料和方法体外淋巴细胞转化试验:应用非特异性刺激物—植物血凝素(PHA),用771培养基加20%自身血浆作培养液,对体外培养的淋巴细胞观察其转化成母细胞的百分率。(PHA冻干粉系广东医药工业研究所出品;771综合培养基系上海东风生化试剂厂出品)。免疫球蛋白(Ig)测定(琼脂单向
From May 1979 to April 1980, 285 patients with schistosomiasis japonica in our hospital were tested for cellular immunity and humoral immunity, and compared with normal people. Meanwhile, the acute cases of serum protein electrophoresis And ring egg reaction, in some cases, after effective drug treatment 2 weeks, 2 months and 4 to 9 months, were followed up. MATERIALS AND METHODS In vitro lymphocyte transformation assay: Using non-specific stimulator-phytohaemagglutinin (PHA), 771 medium plus 20% autologous plasma was used as culture medium to observe the percentage of transformed lymphocytes in vitro . (PHA freeze-dried powder produced by the Guangdong Institute of Pharmaceutical Industry; 771 comprehensive training system Shanghai Dongfeng Biochemical Reagent Factory). Immunoglobulin (Ig) assay (agar unidirectional