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细菌学方法在应用抗菌素治疗的过程中,微生物不仅产生抗药株,同时也产生对抗菌素成为正常发育和生长的必要成份。等(1958)用二个的胆汁枸橼酸培基,其中一个加合霉素,同时进行培养,结果阳性数几乎提高一倍。方法是将100毫克合霉素溶于10毫升酒精中,每100毫升培基,加上述酒精溶液0.5毫升,使1毫升培基含50r合霉素。现将各学者应用结果列表如下:培养阳性率不高,可能是标本中同时存在特异性的噬菌体。(1963)用效价1:28-1:256的多价痢疾噬菌体静脉内免疫家兔,制备有广谱作用的痢疾抗噬菌体血清。在接种前30分钟,滴1-2滴稀释1:10的痢疾抗噬菌体血清于培基的
Bacteriological methods In the course of the application of antibiotics, the microorganisms not only produce resistant strains, but also produce essential ingredients for the normal development and growth of the antibiotics. Et al. (1958) used two bile citrate supplements, one of which added simsomycin and cultured at the same time, resulting in a nearly doubling of positive numbers. The method is to dissolve 100 mg of oxycinomycin in 10 ml of ethanol, and add 0.5 ml of the alcohol solution to each 100 ml of the culture medium so that the 1 ml of the culture medium contains 50 μl of the tobramycin. Now the results of the application of the list of scholars are as follows: culture positive rate is not high, the specimen may be the existence of specific phages. (1963) The rabbits were immunized intravenously with multivalent dysentery bacteriophages having a titer of 1: 28-1: 256 to prepare a broad-spectrum dysentery anti-phage serum. Thirty-two minutes prior to inoculation, drop 1 to 2 drops of the dysentery anti-phage serum diluted 1: 10 in culture medium