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目的检测肿瘤坏死因子-α(TNF-α)在前列腺癌组织中的表达,探讨其与前列腺癌临床病理特征和肿瘤血管生成的关系。方法采用免疫组织化学二步法检测2010年1月-2012年1月20例前列腺增生组织、50例前列腺癌组织中TNF-α蛋白和CD105的表达情况,并计数CD105标记的微血管密度(MVD)。结果免疫组织化学结果显示TNF-α在前列腺癌组织的肿瘤细胞膜及细胞质中表达较高(41.72±8.67),而在前列腺增生组织中表达较低(21.01±3.85),差异有统计学意义(t’=13.990,P<0.001);CD105在前列腺癌中阳性染色定位于肿瘤微血管内皮细胞膜表达较高(20.15±2.67),而在前列腺增生组织中表达较低(4.34±1.67),差异有统计学意义(t’=29.771,P<0.001)。TNF-α和CD105的表达与患者年龄、肿瘤大小无关,与前列腺癌的临床分期呈正相关(rs=0.847,P<0.001;rs=0.776,P<0.001),与病理分级呈负相关(rs=-0.769,P<0.001;rs=-0.842,P<0.001)。结论前列腺癌组织中存在TNF-α的高表达,其可能在前列腺癌的血管生成和肿瘤的发生、发展中起重要作用。
Objective To detect the expression of tumor necrosis factor-α (TNF-α) in prostate cancer and to investigate its relationship with the clinicopathological features and tumor angiogenesis in prostate cancer. Methods Immunohistochemistry was used to detect the expression of TNF-α protein and CD105 in 20 cases of benign prostatic hyperplasia and 50 cases of prostatic carcinoma between January 2010 and January 2012. The expression of CD105-labeled microvessel density (MVD) . Results The results of immunohistochemistry showed that TNF-α was highly expressed in the tumor cell membrane and cytoplasm of prostate cancer (41.72 ± 8.67), but lower in benign prostatic hyperplasia tissues (21.01 ± 3.85), the difference was statistically significant (t ’= 13.990, P <0.001). The positive staining of CD105 in prostate cancer was localized in the membrane of tumor microvascular endothelial cells (20.15 ± 2.67), but lower in benign prostatic hyperplasia tissues (4.34 ± 1.67) Significance (t ’= 29.771, P <0.001). The expression of TNF-α and CD105 was not related with the age of patients and tumor size, but positively correlated with the clinical staging of prostate cancer (rs = 0.847, P <0.001; rs = 0.776, P <0.001) -0.769, P <0.001; rs = -0.842, P <0.001). Conclusion The high expression of TNF-α in prostate cancer tissues may play an important role in the angiogenesis and tumorigenesis of prostate cancer.