论文部分内容阅读
作者将18～20g无品系小鼠分成4组,腹内感染经18小时孵育的绿脓杆菌细胞10亿个(该菌株分离自烧伤患者,对0.5mg/ml艮他霉素敏感)。感染后30～40分钟进行治疗,其中三组分别肌注艮他霉素15、20、50mg/kg,注射液体积为0.3ml,第4组肌注0.3ml生理盐水,作为对照。3～3.5小时后用生理盐水冲洗腹腔,用腹腔冲洗液制作细菌细胞超薄切片,用电镜观察。同时研究了1～2MIIK(最大耐受浓度)的艮他霉素在体外对同一株绿脓杆菌5小时培养物的作用,经3小时孵育后,同样制作超薄切片。
The authors categorized 18-20 g of lineage-free mice into 4 groups and infected 1 billion Pseudomonas aeruginosa cells infected intra-abdominal for 18 hours (this strain was isolated from burns and sensitized to 0.5 mg / ml gentamicin). 30 to 40 minutes after infection for treatment, including three groups of intramuscular injection of gentamycin 15,20,50 mg / kg, the volume of the injection of 0.3ml, intramuscular injection of group 4 0.3ml saline, as a control. After 3 to 3.5 hours, the abdominal cavity was washed with saline and ultrathin sections of bacterial cells were made with peritoneal washings and observed with electron microscope. At the same time, the effect of 1 to 2 MIIK (maximum tolerated concentration) gentamicin on the same 5-hour culture of Pseudomonas aeruginosa in vitro was studied. After 3 hours of incubation, ultrathin sections were similarly prepared.