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对肾性高血压大鼠及经静脉或侧脑室注射血管紧张素Ⅱ(AⅡ)大鼠的观察曾发现,血浆、下丘脑、中脑中央灰质及肾组织中CAMP含量明显升高。为探讨此变化的机理,本文以示踪动力学方法对静注AⅡ大鼠血浆cAMP水平及其代谢清除率(MCR)和产生速率(PR)作了初步观察。材料与方法(一)给药及取样健康成年雄性大鼠,体重240～300克,腹腔麻醉,股静脉插管,全身肝素化后取血O.5毫升,测血浆正常cAMP值。实验组两次静注AⅡ(2μg/200μl和0.5μg/100μl,间隔10分钟),5分
In renal hypertensive rats and intravenous or intracerebroventricular injection of angiotensin Ⅱ (A Ⅱ) observed in rats, plasma, hypothalamus, midbrain gray matter and renal tissue CAMP levels were significantly increased. In order to explore the mechanism of this change, the cAMP level, the metabolic rate (MCR) and the production rate (PR) of plasma in AII rats were measured by tracer kinetics. MATERIALS AND METHODS (I) Administration and Sampling Healthy adult male rats weighing 240-300 grams were anesthetized by intraperitoneal anesthesia and femoral vein cannulation. After systemic heparinization, 0.5 ml of blood was taken to measure the plasma normal cAMP value. Experimental group two intravenous A Ⅱ (2μg / 200μl and 0.5μg / 100μl, interval of 10 minutes), 5 points