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采用PCR-SSCP,免疫组化等方法研究了卵巢癌患者体液中细胞因子的活性与癌基因的表达及其与卵巢癌生物学行为的相关性.结果:一、在卵巢癌,p53变化的阳性率为43.3%;Ⅰ、Ⅱ期者为21.4%,Ⅲ、Ⅳ期者为62.5%(P<0.05),而与组织类型及分级无相关性,但多见于浆液型、低分化肿瘤.卵巢癌中nm23基因产物多为胞浆内表达,阳性表达率为26.67%,阳性表达的恶性程度较低,多属高、中分化,病程短,临床早期的病人,而良性肿瘤及正常卵巢组织多为核内表达.二、卵巢癌患者血清及腹水中细胞因子IL-6 M-CSF水平均明显高于卵巢良性上皮性肿瘤与正常对照组者(P<0.05),尤其是卵巢癌患者腹水中M-CSF水平的升高更为明显(P<0.01),而卵巢良性上皮性肿瘤与正常对照组相比,无明显差
The activity of cytokines and the expression of oncogenes in ovarian cancer patients and their correlation with the biological behavior of ovarian cancer were studied by PCR-SSCP and immunohistochemistry.Results: 1. In ovarian cancer, the positive changes of p53 The rate was 43.3% in stage Ⅰ and stage Ⅱ patients, 21.4% in stage Ⅰ and stage Ⅱ, and 62.5% in stage Ⅲ and Ⅳ (P <0.05), but not related to the type and grade of tissue, but was more common in serous and poorly differentiated tumors. Most of the nm23 gene products were expressed in the cytoplasm, the positive expression rate was 26.67%. The positive expression rate of the nm23 gene was low, most of which were high, moderately differentiated, with short course of disease and early clinical stage, while the benign tumors and normal ovarian tissues were mostly The levels of IL-6 and M-CSF in serum and ascites of patients with ovarian cancer were significantly higher than those of benign ovarian epithelial tumors and normal controls (P <0.05), especially in patients with ovarian cancer -CSF level increased more significantly (P <0.01), while benign ovarian epithelial tumors compared with the normal control group, no significant difference