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建立以N,N-二甲基乙二胺作为柱前衍生试剂,在C18反相色谱柱上,采用梯度洗脱,电喷雾电离源测定羊栖菜、紫菜、海带中饱和脂肪酸和不饱和脂肪酸的柱前衍生高效液相色谱串联质谱法。各脂肪酸衍生物均在进样质量浓度为2~100μg/m L时表现出良好的线性关系,相关系数均≥0.9905。检出限(LODs)在20~50 ng/m L之间,方法比未衍生脂肪酸的方法提高了1000倍左右的灵敏度。
To establish a pre-column derivatization reagent with N, N-dimethylethylenediamine, and to determine the saturated fatty acids and unsaturated fatty acids in Sargassum fusiforme, seaweed and kelp on a C18 reversed-phase column by gradient elution and electrospray ionization Pre-Column Derivatization High Performance Liquid Chromatography Tandem Mass Spectrometry. Each fatty acid derivative showed good linearity when the injection mass concentration was 2 ~ 100μg / m L, the correlation coefficients were both ≥0.9905. The limits of detection (LODs) ranged from 20 ng / mL to 50 ng / mL. The method was a factor of about 1000-fold more sensitive than non-derivatized fatty acids.