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采用石墨烯作为吸附剂制备固相萃取(SPE)小柱,结合高效液相色谱用于环境水样中痕量罗丹明B的灵敏检测。由于大比表面积的石墨烯与罗丹明B之间具有强烈的π-π共轭作用,可提高罗丹明B在石墨烯上的吸附量,从而增强所制备的石墨烯基SPE小柱对罗丹明B的富集能力。对固相萃取条件进行优化,在优化的条件下采用Inertsil ODS-SP(250 mm×4.6 mm,5μm)色谱柱,水:甲醇=25:75(V/V)做为流动相,流速1.0 m L/min,进样量20μL,550 nm波长下进行检测。该方法在3.91~250.0μg/L范围内线性关系良好,检出限(3σ)为2.29μg/L。该SPE小柱对罗丹明B具有良好的选择性富集能力,可以实现环境水样中痕量罗丹明B的准确和灵敏检测。
Graphene was used as adsorbent to prepare solid-phase extraction (SPE) cartridges, coupled with high performance liquid chromatography (HPLC) for the sensitive detection of trace rhodamine B in environmental water samples. Due to the strong π-π conjugation between graphene and rhodamine B with large specific surface area, the adsorption capacity of rhodamine B on graphene can be increased, and the adsorption of Rhodamine B’s enrichment ability. The conditions of solid-phase extraction (SPE) were optimized with Inertsil ODS-SP (250 mm × 4.6 mm, 5 μm) column under the optimized conditions. The mobile phase consisted of water: methanol = 25:75 L / min, injection volume 20μL, 550 nm wavelength detection. The method has a good linearity in the range of 3.91 ~ 250.0μg / L with a detection limit (3σ) of 2.29μg / L. The SPE cartridge has good selective enrichment ability for rhodamine B, and can accurately and sensitively detect trace rhodamine B in environmental water samples.