具抗肿瘤活性海洋细菌HY108的发酵优化

• 作 者: 张卫 田树红 张云波 马瑞莲
• 机 构: 三亚市人民医院药物临床试验中心,
• 出 处: 中国热带医学
• 发表日期: 2012年02期
• 关键词: 海洋细菌 MTT法 抗肿瘤 发酵优化

目的对海洋细菌HY108进行发酵工艺的优化,以提高菌株发酵液的抗肿瘤活性。方法采用MTT检测法检测菌株发酵液对人肝癌细胞SMMC-7721的抑制率,对该菌株的培养基组成和发酵条件进行了确定。结果通过对该菌株产抗肿瘤活性物质的发酵培养基和发酵条件的优化,菌株发酵液对肿瘤细胞的抑制率提高了近20%,为提高抗肿瘤先导化合物的产率提供了理论依据。结论通过优化,获得最佳发酵培养基为:玉米粉3%、葡萄糖0.5%、蛋白胨0.2%和酵母粉1.2%,pH6.5～7.0;最佳发酵条件为:种龄24h,以2%的接种量接种,28℃的条件下150r/min振荡培养3d。

Objective To optimize the fermentation process of marine bacterium HY108 to improve the anti-tumor activity of the strain fermentation broth. Methods MTT assay was used to determine the inhibitory rate of the fermentation broth to human hepatocellular carcinoma cell line SMMC-7721. The medium composition and fermentation conditions of the strain were determined. Results By optimizing the fermentation medium and fermentation conditions of the strain producing antitumor active substances, the inhibitory rate of the strain fermentation broth on tumor cells was increased by nearly 20%, providing a theoretical basis for improving the yield of antitumor lead compounds. Conclusion The optimized fermentation medium is as follows: 3% cornstarch, 0.5% glucose, 0.2% peptone and 1.2% yeast, pH6.5 ~ 7.0. The optimum fermentation conditions are as follows: Vaccination inoculation, 28 ℃ under the conditions of 150r / min shaking culture 3d.