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以光活化菊酯农药新理念为指导,设计合成了氯丙炔菊酯的二炔衍生物(JZB),并以胺菊酯、溴氰菊酯、联苯菊酯和氯丙炔菊酯为对照药剂,采用MTT法、Giemsa染色法和流式细胞术研究JZB对斜纹夜蛾Spodopteralitura(SL)细胞的光活化毒力和毒性机制。结果表明,500μg/mL处理SL细胞后24h,氯丙炔菊酯及JZB均表现出良好的抑制活性,抑制率分别达89.79%和92.95%,而胺菊酯、溴氰菊酯和联苯菊酯的抑制率均低于21%;氯丙炔菊酯及JZB对SL细胞形态影响明显,50μg/mL处理后24h,细胞密度降低,90%以上的细胞收缩变圆,无法正常贴壁,而500μg/mL溴氰菊酯处理后24h,70%以上细胞贴壁状态良好;经50μg/mL氯丙炔菊酯及JZB处理后,细胞线粒体膜电位较对照分别降低了35.43%和45.55%,活性氧较对照分别升高212.09%和311.60%。
Under the guidance of the new concept of photoactivated pyrethrin pesticides, the diyne derivative (JZB) of chlorpromazine was designed and synthesized, and the reaction of tetramethrin, deltamethrin, bifenthrin and chlorpromazine was The control agents, the MTT method, Giemsa staining and flow cytometry JZB Spodopteralitura (SL) cells of light-induced toxicity and toxicity mechanisms. The results showed that both clopidogrel and JZB showed good inhibitory activity at the rate of 89.79% and 92.95% respectively after treated with 500μg / mL for SL cells. However, tetramethrin, deltamethrin and bifenthrin The inhibitory rate of ester was less than 21%. Chlorpromazine and JZB had obvious effects on the morphology of SL cells. After 50μg / mL treatment, the cell density decreased and more than 90% of the cells became contracted. After 500μg / mL deltamethrin treatment, more than 70% of the cells adhered well, and the mitochondrial membrane potential decreased by 35.43% and 45.55% respectively after 50μg / mL CPP and JZB treatment, the activity Oxygen increased 212.09% and 311.60% respectively compared with the control.