论文部分内容阅读
目的探讨脊髓水通道蛋白1(aquaporin 1,AQP1)在大鼠坐骨神经慢性压迫性损伤(CCI)所致神经病理性疼痛中的作用。方法雄性SD大鼠25只,随机分为5组,每组5只:A组:大鼠建立CCI模型后观察4天;B组:大鼠建立CCI模型后观察7天;C组:大鼠建立CCI模型后观察14天;D组:正常对照组,正常饲养观察16天(术前2天+术后观察14天);E组:假手术组:暴露左侧坐骨神经中段仅绕线,不结扎,术后观察14天。各组大鼠在建模前1天、建模后第1、4、7、14天测量所有未处死大鼠双后肢的热痛阈(PWTL)和机械痛阈(PWMT)。达到观察时间取大鼠L4~5节段脊髓,免疫组化法观察AQP1在脊髓的定位分布和表达变化,Realtime PCR法检测脊髓AQP1的mRNA表达变化。结果与同组右侧脊髓背角比较,和C组大鼠左侧脊髓背角AQP1阳性颗粒的平均光密度和面积增高(P<0.05)。A、B、C组大鼠的脊髓背角AQP1阳性颗粒的面积高于正常对照组和假手术组(P<0.05),D组与E组之间无差别(P>0.05)。与正常组和假手术组比较,A、B和C组大鼠脊髓AQP1 mRNA含量显著增高(P<0.05),D组与E组之间无差异(P>0.05),A组、B组、C组大鼠脊髓AQP1的mRNA含量分别为D组含量的1.688、4.876和5.806倍。结论大鼠脊髓AQP1可能参与神经病理性疼痛的形成和发展。
Objective To investigate the role of aquaporin 1 (AQP1) in neuropathic pain induced by chronic constriction injury (CCI) in rat sciatic nerve. Methods Twenty-five male Sprague-Dawley rats were randomly divided into 5 groups with 5 rats in each group: Group A: CCI model was established in rats and observed for 4 days; Group B: CCI model was observed in rats for 7 days; Group C: The CCI model was observed after 14 days. Group D: Normal control group, 16 days after normal feeding (2 days before operation and 14 days after operation); Group E: Sham operation group: only the middle part of sciatic nerve exposed only wound, Ligation, postoperative observation of 14 days. The thermal pain threshold (PWTL) and mechanical pain threshold (PWMT) of all the rats in each group were measured on the 1st day before modeling and on the 1st, 4th, 7th and 14th days after modeling. At the time of observation, the spinal cord was taken from the L4 ~ 5 segments of spinal cord in rats. The distribution and expression of AQP1 in spinal cord were observed by immunohistochemistry. The mRNA expression of AQP1 in spinal cord was detected by Realtime PCR. Results Compared with the right dorsal horn in the same group, the mean optical density and area of AQP1 positive particles in the left spinal cord of C group were significantly increased (P <0.05). The area of AQP1 positive particles in the dorsal horn of spinal cord in group A, B and C was higher than that in normal control group and sham operation group (P <0.05). There was no difference between group D and group E (P> 0.05). Compared with normal group and sham operation group, AQP1 mRNA content in spinal cord of A, B and C groups was significantly increased (P <0.05), there was no significant difference between D group and E group (P> 0.05) The mRNA contents of AQP1 in spinal cord of group C were 1.688, 4.876 and 5.806 times of that of group D, respectively. Conclusion AQP1 in rat spinal cord may be involved in the formation and development of neuropathic pain.