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用从出生２４小时的乳鼠长骨中分离出来的破骨细胞，从１月龄大鼠长骨中分离出来的骨髓基质细胞，建立破骨细胞培养和破骨细胞、骨髓基质细胞培养系。在白细胞介素１（ＩＬ１α）作用的同时，加入不同剂量的密骨片药液，分别与牛骨片共同培养２０小时。计数骨片形成的陷窝数及测算吸收陷窝面积。并采用原位杂交技术，研究密骨片对破骨细胞基质金属蛋白酶９（ＭＭＰ９）ｍＲＮＡ表达的调控。结果显示，在基质细胞存在的条件下，ＩＬ１可使骨吸收性增强２～４倍，ＭＭＰ９ｍＲＮＡ呈高表达。密骨片则可对抗ＩＬ１对基质细胞的影响，间接地抑制破骨细胞活性，使ＭＭＰ９ｍＲＮＡ表达降低，减少骨吸收。
Osteoclast cultures and cultures of osteoclasts and bone marrow stromal cells were established from bone marrow stromal cells isolated from one-month-old rat long bones using osteoclasts isolated from the long bones of neonatal rat born for 24 hours. In the interleukin 1 (IL 1α) role at the same time, add different doses of bone marrow tablets, were incubated with bovine bone chips together for 20 hours. The number of lacunae formed by the bone fragments was counted and the absorptive lacune area was calculated. In situ hybridization was used to study the regulation of osteoblast MMP-9 mRNA expression in osteoclasts. The results showed that in the presence of stromal cells, IL-1 enhanced bone resorption by 2 to 4 fold, and MMP-9 mRNA was highly expressed. The mechanoids can counteract the effects of IL-1 on stromal cells, indirectly inhibit the activity of osteoclasts, reduce the expression of MMP-9 mRNA, and reduce bone resorption.