论文部分内容阅读
应用十六烷基三甲基溴化铵(CTAB)作为表面活性剂修饰单壁碳纳米管,研究CTAB修饰后的单壁碳纳米管的分散情况和表面电荷情况;观察在场发射扫描电子显微镜和透射电子显微镜下的形貌,同时还研究CTAB修饰后的单壁碳纳米管与小干扰RNA结合的最佳浓度配比,以及CTAB功能修饰后的碳纳米管对培养的人脐静脉内皮细胞的毒性。结果表明:CTAB修饰后的单壁碳纳米管分散良好,CTAB吸附到单根或成束的碳纳米管管壁上,表面带正电荷;与带负电荷的寡核苷酸分子小干扰RNA可以结合,并且CTAB-SWNT与小干扰RNA结合比例达到1:1.5到1:2之间时基本饱和;缺乏CTAB的单壁碳纳米管不能结合小干扰RNA;没有分散的单壁碳纳米管具有更大的细胞毒性,CTAB可以改善SWNTs的分散性,从而减轻单壁碳纳米管的细胞毒性。
Single-walled carbon nanotubes (CTABs) were modified with cetyltrimethylammonium bromide (CTAB) as surfactant to investigate the dispersion and surface charge of CTAB-modified single-wall carbon nanotubes (MWCNTs) Transmission electron microscopy morphology, but also to study the CTAB modified single-walled carbon nanotubes and small interfering RNA binding the best concentration ratio, and CTAB functional modified carbon nanotubes cultured human umbilical vein endothelial cells toxicity. The results showed that the single-walled carbon nanotubes modified by CTAB were dispersed well, and the CTAB was adsorbed onto the wall of single-walled or bundled carbon nanotubes with a positive charge on the surface. The small interfering RNA , And the ratio of CTAB-SWNT to small interfering RNA is essentially saturated between 1: 1.5 and 1: 2; single-walled carbon nanotubes lacking CTAB do not bind to small interfering RNA; undispersed single-walled carbon nanotubes have more Large cytotoxicity, CTAB can improve the dispersion of SWNTs, thereby reducing the single-walled carbon nanotubes cytotoxicity.