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目的:应用血清药理学方法研究千里光抗大肠埃希菌的作用及其机制。方法:从千里光中分离出黄酮类化合物,然后通过给小鼠灌胃制备千里光水浸液的含药血清和黄酮类化合物的含药血清。用扫描电镜观察各含药血清对大肠埃希菌超微结构的影响,用同位素前体参入试验观察各含药血清对大肠埃希菌生物合成的影响。结果:与阴性对照血清相比,2种含药血清作用后的大肠埃希菌,其形态和超微结构均发生明显的变化,表现为菌体表面粗糙,部分菌体出现缢痕,并在缢痕处缢缩或断裂等变化;DNA、RNA、蛋白质和肽聚糖合成也受到明显的抑制。结论:千里光的抗大肠埃希菌作用机制可能是通过抑制细菌的DNA、RNA、蛋白质和肽聚糖的合成有关,其作用的有效成份可能是黄酮类化合物。
OBJECTIVE: To study the effect and mechanism of Senecio ophthalmic Escherichia coli using serum pharmacology. Methods: Flavonoids were isolated from Senecio, and the medicated serum and the flavonoid-containing serum of Serengeti were collected by intragastric administration to mice. Scanning electron microscopy was used to observe the effect of each drug-containing serum on the ultrastructure of Escherichia coli. The isotope precursor incorporation assay was used to observe the effect of each drug-containing serum on the biosynthesis of Escherichia coli. Results: Compared with the negative control serum, the morphology and ultrastructure of Escherichia coli after 2 kinds of drug-containing serum were changed obviously. The results showed that the surface of the cell was rough, Constriction shrinkage or fracture change; DNA, RNA, protein and peptidoglycan synthesis was also significantly inhibited. Conclusion: The anti-escherichia coli mechanism of Senecio may be related to the inhibition of bacterial DNA, RNA, protein and peptidoglycan synthesis. The active ingredient may be flavonoids.