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目的:观察黄连解毒汤(HLJDT)对自发性高血压大鼠(SHR)血压及其内皮祖细胞(EPCs)一氧化氮(NO)分泌功能的影响,并探讨其作用机制。方法:60只雄性12周龄SHR随机分为5组,分别为模型组,卡托普利阳性药组,HLJDT低、中、高剂量组,每组12只,12只同周龄雄性WKY大鼠作为正常组。正常组和模型组予生理盐水ig(20 m L·kg~(-1)),其余各组分别给予卡托普利ig(1.35 mg·kg~(-1)),HLJDT低剂量ig(13.5 mg·kg~(-1)),HLJDT中剂量ig(27 mg·kg~(-1)),HLJDT高剂量ig(54mg·kg~(-1)),均1次/d,连续2周。用药前及预处理结束后当天采用BP-6大鼠无创血压测试仪测定各组大鼠尾动脉收缩压,采用密度梯度离心法分离培养骨髓来源的EPCs,经Dil-ac-LDL和FITC-UEA-I双染色鉴定后,分别采用细胞计数试剂盒(CCK-8)检测法、迁移小室(Transwell)小室、黏附实验及NO检测分析各组EPCs增殖、迁移、黏附能力及NO分泌功能。结果:与正常组比较,模型组SHR血压明显升高,SHR内皮祖细胞数量,增殖能力,迁移能力,黏附能力及NO分泌功能明显降低(P<0.01);与模型组比较,HLJDT可以降低SHR血压,HLJDT低、中、高剂量组SHR内皮祖细胞数量,增殖能力,迁移能力,黏附能力及NO分泌功能均有增加(P<0.05,P<0.01)。结论:HLJDT能降低SHR血压,并且能增加EPCs的数量,提高EPCs的增殖、迁移、黏附能力及NO分泌功能。
Objective: To observe the effect of HLJDT on the blood pressure and the secretion of nitric oxide (NO) in spontaneously hypertensive rats (SHR) and its mechanism of action. Methods: Sixty male 12-week-old SHRs were randomly divided into 5 groups: model group, captopril positive drug group, HLJDT low, medium and high dose group, 12 rats in each group, 12 rats of the same age with WKY Rats as normal group. The rats in normal group and model group were treated with ig (20 m L · kg -1) of physiological saline, and the other groups were given captopril ig (1.35 mg · kg -1), HLJDT low dose ig mg · kg -1, HLJDT medium dose ig (27 mg · kg -1) and HLJDT high dose ig (54 mg · kg -1), once a day for 2 weeks . BP-6 rat noninvasive blood pressure tester was used to measure the tail artery systolic pressure before and after the pretreatment. The bone marrow-derived EPCs were isolated and cultured by density gradient centrifugation. Dil-ac-LDL and FITC-UEA -I double staining. The proliferation, migration, adhesion and NO secretion of EPCs were detected by CCK-8 assay, transwell chamber assay, adhesion assay and NO assay respectively. Results: Compared with the normal group, the blood pressure of SHR in model group was significantly increased, and the number of EPCs, the ability of proliferation, migration, adhesion and NO secretion of SHR were significantly decreased (P <0.01). Compared with model group, HLJDT could reduce SHR Blood pressure, HLJDT low, medium and high dose SHR EPC number, proliferation, migration, adhesion and NO secretion increased (P <0.05, P <0.01). CONCLUSION: HLJDT can reduce the blood pressure of SHR and increase the number of EPCs, and increase the proliferation, migration, adhesion and NO secretion of EPCs.