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目的:探讨~(18)F-FDG摄取和肿瘤内癌基因表达之间的关系。方法:10只成瘤裸鼠随机分为Hep-2组和HIF-RNAi-Hep-2组,每组5只。对两组动物进行~(18)F-FDG PET扫描,计算移植瘤T/N比值。PET扫描结束,剥取肿瘤组织进行免疫组织化学检测。结果:Hep-2组和HIF-RNAi-Hep-2组肿瘤PET显像均清晰、明显,经统计学分析,2组动物移植瘤T/N比值差异无统计学意义(P>0.05)。移植瘤内癌基因HIF-1α、Glut-1和VEGF在Hep-2组表达明显,在HIF-RNAi-Hep-2组HIF-1α表达明显减弱,两组之间差异有统计学意义(P<0.05)。而Glut-1和VEGF在HIF-RNAi-Hep-2组高表达,与Hep-2组相比,差异无统计学意义(P>0.05)。结论:喉鳞状细胞癌移植瘤~(18)F-FDG摄取和肿瘤内部癌基因通路的激活密切相关,其中糖酵解以及血管生成相关基因可能起到关键作用。
Objective: To investigate the relationship between ~ (18) F-FDG uptake and oncogene expression in tumor. Methods: Ten tumor-bearing nude mice were randomly divided into Hep-2 group and HIF-RNAi-Hep-2 group, with 5 mice in each group. The two groups of animals were subjected to ~ (18) F-FDG PET scan to calculate the T / N ratio of the transplanted tumor. PET scanning was completed, stripped of tumor tissue for immunohistochemistry. Results: The PET imaging of Hep-2 and HIF-RNAi-Hep-2 tumors was clear and obvious. There was no significant difference in T / N ratio between the two groups (P> 0.05) by statistical analysis. The expression of oncogene HIF-1α, Glut-1 and VEGF in Hep-2 group was significantly higher than that in HIF-RNAi-Hep-2 group, and the difference was statistically significant (P < 0.05). The expression of Glut-1 and VEGF in HIF-RNAi-Hep-2 group was significantly higher than that in Hep-2 group (P> 0.05). CONCLUSION: The uptake of 18F-FDG in the transplanted laryngeal squamous cell carcinoma is closely related to the activation of oncogene pathways in the tumor. Among them, glycolytic and angiogenesis related genes may play key roles.